Localization of inducible nitric oxide synthase (iNOS) protein in acetic acid-induced gastric rat ulcers. The localization of rat iNOS-positive cells after ulcer induction using acetic acid was immunohistochemically examined. (a) Haematoxylin and eosin staining of a specimen obtained 24 h after ulcer induction. (b) iNOS immunostaining of specimens obtained 24 h after ulcer induction, (c) 72 h after ulcer induction and (e) 7 days after ulcer induction. (d) Higher magnification of the specimen shown in c (original magnification of a, b, c and e, ×100; d, ×200). Arrows indicate iNOS-positive cells.