Figure 6.

Temporal changes in the expression of Bcl-2- and Bax-proteins and the release of cytochrome C from mitochondria to the cytosol. Temporal changes in the expression of Bcl-2- and Bax-proteins and the release of cytochrome C from mitochondria to the cytosol in the cells treated with NOC-18, under low-glucose condition, were analysed by Western blotting. Bax protein was not detected in the control (time zero), but its expression increased gradually after treatment with NOC-18 (the first rank). The protein expression of Bcl-2 did not show any change compared with the control (the second rank). Western blotting for cytochrome C was performed using individual protein samples from either mitochondrial fractions (Mito, the third rank) or cytosolic fractions (Cyto, the fourth rank). In the control, cytochrome C was detected only in the mitochondrial fraction. After treatment with NOC-18, it weakly appeared in the cytosolic fraction, 6 h later, and was gradually increasing. The density of cytochrome C immunoreactants was ultimately inverted between both fractions, 24 h later.