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. Author manuscript; available in PMC: 2008 Aug 18.
Published in final edited form as: J Proteome Res. 2006 Jul;5(7):1636–1646. doi: 10.1021/pr0502469

Figure 2. Phosphoryaltion of Mesangial Lysate by Recombinant Active Akt.

Figure 2

Candidate Akt substrates were identified by phosphorylation of mesangial lysate with recombinant active Akt in the presence of [γ-P32]ATP. Proteins were separated by 2-DE, and phosphoproteins were detected by autoradiography. Phosphoproteins were identified by comparing autoradiographs with corresponding Sypro-Ruby-stained gels. Five candidate Akt substrates identified by peptide mass fingerprinting of trypsin-digested phosphoproteins using MALDI-MS are indicated by arrows.