Table 1.
List of candidate Akt Substrates identified by incubation of mesangial cell lysate with active recombinant Akt in an in vitro kinase assay or immunoblot (IB) analysis of lysate from PDGF-stimulated cells.
|
Method of Separation |
||||
|---|---|---|---|---|
| Functional Category | Protein | gi | SDS-PAGE | 2-DE |
| Chaperone | Hsp84 | 51890229 | Kinase Assay | |
| Hsp86 | 54673763 | Kinase Assay | ||
| Hsp70 | 56385 | IB | ||
| Hsp27 | 14010865 | Kinase Assay & IB | ||
| Grp94 | 34862435 | IB | ||
| Grp78 | 25742763 | IB | Kinase Assay | |
| Grp58 | 8393322 | IB | ||
| PDI | 6981324 | IB | ||
| Cytoskeletal | actin, beta | 71620 | Kinase Assay & IB | |
| Vimentin | 14389299 | Kinase Assay & IB | ||
| tropomyosin 5 | 9653293 | Kinase Assay | ||
| tubulin, beta chain | 92930 | IB | ||
| Similar to FLJ00343 protein (filamin) | 34881882 | IB | ||
| myosin heavy chain | 6981236 | IB | ||
| lamin A | 1072002 | Kinase Assay | ||
| Glycolytic | Enolase | 6978809 | Kinase Assay | |
| Aldolase A | 6978487 | IB | ||
| Phospholipid Binding | Annexin I | 6978501 | Kinase Assay | |
| ATP Synthesis | F1-ATP synthase β-subunit | 92350 | Kinase Assay | |
| Adaptor Protein | 14−3−3 ε | 13928824 | IB | |