Table 2.
Identification of HSP90α, HSP90β, HSP70, GRP78, GRP94, and PDI as Akt Substrates by peptide mass fingerprinting.
| Protein | NCBInr Entry | Matched Peptidesa | Coverage (%)b | Mascot Score | Matched Massc | Startd | Ende | Peptide Sequencef |
|---|---|---|---|---|---|---|---|---|
| Hsp90β | 51890229 | 16/36 | 27 | 178 | 1274.64 | 42 | 53 | ELISNASDALDK |
| 2255.01 | 149 | 168 | HNDDEQYAWESSAGGSFTVR | |||||
| 950.46 | 169 | 177 | ADHGEPIGR | |||||
| 1310.56 | 187 | 196 | EDQTEYLEER | |||||
| 1150.55 | 276 | 284 | YIDQEELNK | |||||
| 900.52 | 285 | 291 | TKPIWTR | |||||
| 1526.74 | 307 | 319 | SLTNDWEDHLAVK | |||||
| 1347.66 | 320 | 330 | HFSVEGQLEFR | |||||
| 828.52 | 331 | 337 | ALLFIPR | |||||
| 1512.78 | 379 | 392 | GVVDSEDLPLNISR | |||||
| 1140.55 | 439 | 448 | LGIHEDSTNR | |||||
| 2175.94 | 457 | 475 | YHTSQSGDEMTSLSEYVSR | |||||
| 1248.61 | 492 | 502 | EQVANSAFVER | |||||
| 2431.1 | 584 | 604 | LVSSPCCIVTSTYGWTANMER | |||||
| 1263.49 | 613 | 623 | DNSTMGYMMAK | |||||
| 1781.94 | 625 | 639 | HLEINPDHPIVETLR | |||||
| Peptides not matched: 662.42, 663.41, 706.56, 714.38, 732.52, 742.42, 768.46 1168.65, 1194.68, 1208.65, 1236.68, 1364.78, 1515.84, 1541.78, 1778.91, 1786.99, 1790.95, 1911.07, 2374.21, 2447.15 | ||||||||
| Hsp90α | 54673763 | 12/36 | 18 | 109 | 2254.95 | 154 | 173 | HNDDEQYAWESSAGGSFTVR |
| 1310.56 | 192 | 201 | EDQTEYLEER | |||||
| 1777.94 | 210 | 224 | HSQFIGYPITLFVEK | |||||
| 1150.55 | 285 | 293 | YIDQEELNK | |||||
| 900.52 | 294 | 300 | TKPIWTR | |||||
| 1540.75 | 316 | 328 | SLTNDWEEHLAVK | |||||
| 1347.66 | 329 | 339 | HFSVEGQLEFR | |||||
| 1512.78 | 388 | 401 | GVVDSEDLPLNISR | |||||
| 1167.56 | 448 | 457 | LGIHEDSQNR | |||||
| 713.28 | 480 | 484 | DYCTR | |||||
| 1207.62 | 491 | 500 | HIYFITGETK | |||||
| 1785.94 | 634 | 648 | HLEINPDHSIIETLR | |||||
| Peptides not matched: 662.42, 663.41, 706.56, 732.52, 742.42, 768.46, 829.55, 951.53, 1141.61, 1194.68, 1236.68, 1249.67, 1264.67, 1275.74, 1364.78, 1515.84, 1527.84, 1782.98, 1790.95, 1911.07, 2177.00, 2374.21, 2432.18, 2447.15 | ||||||||
| Grp78 | 25742763 | 20/48 | 38 | 233 | 1227.62 | 50 | 60 | VEIIANDQGNR |
| 1565.77 | 61 | 74 | ITPSYVAFTPEGER | |||||
| 1676.8 | 82 | 96 | NQLTSNPENTVFDAK | |||||
| 1603.86 | 124 | 138 | TKPYIQVDIGGGQTK | |||||
| 1551.79 | 139 | 152 | TFAPEEISAMVLTK | |||||
| 1886.96 | 165 | 181 | VTHAVVTVPAYFNDAQR | |||||
| 1232.62 | 186 | 197 | DAGTIAGLNVMR | |||||
| 1658.89 | 198 | 213 | IINEPTAAAIAYGLDK | |||||
| 918.46 | 262 | 268 | VMEHFIK | |||||
| 996.51 | 298 | 306 | ALSSQHQAR | |||||
| 2147.99 | 307 | 324 | IEIESFFEGEDFSETLTR | |||||
| 917.47 | 345 | 352 | VLEDSDLK | |||||
| 1459.75 | 354 | 367 | SDIDEIVLVGGSTR | |||||
| 1835.93 | 448 | 464 | SQIFSTASDNQPTVTIK | |||||
| 1190.63 | 465 | 474 | VYEGERPLTK | |||||
| 1933.01 | 475 | 492 | DNHLLGTFDLTGIPPAPR | |||||
| 1073.55 | 524 | 532 | ITITNDQNR | |||||
| 985.51 | 533 | 540 | LTPEEIER | |||||
| 1315.63 | 563 | 573 | NELESYAYSLK | |||||
| 1396.78 | 622 | 633 | ELEEIVQPIISK | |||||
| Peptides not matched:728.34, 743.26, 753.34, 788.34, 856.45, 870.46, 887.43, 894.41, 959.46, 976.44, 1104.50, 1182.57, 1211.55, 1265.61, 1359.69, 1481.78, 1488.72, 1512.78, 1528.71, 1588.85, 1693.78, 1703.89, 1719.87, 1815.97, 2016.07, 2082.03, 2163.06, 2839.38 | ||||||||
| PDI | 6981324 | 16/38 | 32 | 194 | 861.46 | 59 | 66 | ALAPEYAK |
| 1779.83 | 83 | 98 | VDATEESDLAQQYGVR | |||||
| 695.32 | 116 | 121 | EYTAGR | |||||
| 1050.5 | 215 | 223 | NNFEGEITK | |||||
| 1964.04 | 232 | 248 | HNQLPLVIEFTEQTAPK | |||||
| 1080.67 | 256 | 264 | THILLFLPK | |||||
| 869.38 | 265 | 272 | SVSDYDGK | |||||
| 1832.91 | 287 | 301 | ILFIFIDSDHTDNQR | |||||
| 965.56 | 302 | 309 | ILEFFGLK | |||||
| 859.39 | 311 | 317 | EECPAVR | |||||
| 1221.62 | 318 | 327 | LITLEEEMTK | |||||
| 1408.67 | 328 | 339 | YKPESDELTAEK | |||||
| 1515.73 | 340 | 351 | ITQFCHHFLEGK | |||||
| 1226.55 | 377 | 386 | NFEEVAFDEK | |||||
| 927.52 | 438 | 445 | VHSFPTLK | |||||
| 909.43 | 446 | 453 | FFPASADR | |||||
| Peptides not matched: 751.35, 758.33, 774.33, 808.40, 988.48, 994.12, 1002.53, 1016.52, 1067.50, 1118.50, 1179.59, 1199.65, 1234.58, 1243.66, 1287.70, 1308.64, 1424.70, 1475.74, 1486.71, 1493.73, 1729.87, 1794.83 | ||||||||
| Grp94 | 34862435 | 25/46 | 33 | 277 | 960.45 | 44 | 51 | TDDEVVQR |
| 1784.89 | 52 | 67 | EEEAIQLDGLNASQIR | |||||
| 1080.54 | 76 | 84 | FAFQAEVNR | |||||
| 962.58 | 88 | 95 | LIINSLYK | |||||
| 676.39 | 98 | 102 | EIFLR | |||||
| 1274.64 | 103 | 114 | ELISNASDALDK | |||||
| 2029.06 | 117 | 135 | LISLTDENALAGNEELTVK | |||||
| 1512.77 | 143 | 156 | NLLHVTDTGVGMTR | |||||
| 944.59 | 244 | 252 | GTTITLVLK | |||||
| 1524.72 | 253 | 265 | EEASDYLELDTIK | |||||
| 1186.67 | 385 | 395 | SILFVPTSAPR | |||||
| 1014.47 | 396 | 404 | GLFDEYGSK | |||||
| 782.43 | 429 | 434 | YLNFVK | |||||
| 1484.75 | 435 | 448 | GVVDSDDLPLNVSR | |||||
| 882.46 | 449 | 455 | ETLQQHK | |||||
| 1138.57 | 494 | 503 | LGVIEDHSNR | |||||
| 2249.03 | 512 | 530 | FQSSHHSTDITSLDQYVER | |||||
| 1030.49 | 538 | 546 | IYFMAGSSR | |||||
| 1149.52 | 548 | 557 | EAESSPFVER | |||||
| 753.32 | 598 | 603 | FDESEK | |||||
| 658.38 | 634 | 639 | AVVSQR | |||||
| 2355.14 | 640 | 660 | LTESPCALVASQYGWSGNMER | |||||
| 865.38 | 664 | 671 | AQAYQTGK | |||||
| 1288.59 | 672 | 682 | DISTNYYASQK | |||||
| 875.45 | 684 | 690 | TFEINPR | |||||
| Peptides not matched: 856.45, 870.46, 890.42, 987.46, 1004.48, 1045.53, 1278.62, 1378.66, 1413.65, 1417.71, 1574.84, 1602.77, 1663.88, 1734.74, 1799.94, 2044.09, 2200.11, 2225.14, 2300.15, 2839.41 | ||||||||
| Hsp70 | 56385 | 10/19 | 22 | 96 | 1227.62 | 26 | 36 | VEIIANDQGNR |
| 1486.69 | 37 | 49 | TTPSYVAFTDTER | |||||
| 1664.78 | 57 | 71 | NQVAMNPTNTVFDAK | |||||
| 1980.99 | 138 | 155 | TVTNAVVTVPAYFNDSQR | |||||
| 1198.67 | 160 | 171 | DAGTIAGLNVLR | |||||
| 1658.89 | 172 | 187 | IINEPTAAAIAYGLDK | |||||
| 1690.71 | 221 | 236 | STAGDTHLGGEDFDNR | |||||
| 1252.61 | 302 | 311 | FEELNADLFR | |||||
| 2773.32 | 424 | 447 | QTQTFTTYSDNQPGVLIQVYEGER | |||||
| 1196.66 | 459 | 469 | FELTGIPPAPR | |||||
| Peptides not matched: 709.34, 1029.67, 1057.69, 1082.55, 1145.64 1211.71, 1457.86, 1593.03, 2169.09 | ||||||||
a
number of trypsin-generated peptides matched to identified protein
b
Percentage of protein covered by matched peptide
c
mass of matched peptide
d
start and end positions of matched peptide, respectively
e
start and end positions of matched peptide, respectively
f
corresponding peptide sequence