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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: J Nutr. 2008 Jul;138(7):1274–1281. doi: 10.1093/jn/138.7.1274

FIGURE 5.

FIGURE 5

Dose effects of 24-h treatment with hesperetin or nobiletin on transcriptional activity of the LDLR promoter sequence from -142 to +35 (A) and reduction of flavonoid responsiveness by mutation of the SRE (B). Luciferase values were normalized and expressed as a fold of the corresponding untreated sample as described in the Online Supporting Material. Error bars are ±SEM. (A) Transfected HepG2 cells were treated with hesperetin (n = 4-6 at each dose) or nobiletin (n = 5-7 at each dose) over a range of concentrations. *Different from the untreated control, P ≤ 0.05 (Dunnett’s test). (B) The SREBP binding site was either the native sequence (wt) or mutated at a single nucleotide (mut) (n = 4). *Different between the mut and the wt constructs for each compound at P ≤ 0.0001 (ANOVA).