Skip to main content
. 2008 Sep;10(9):949–953. doi: 10.1593/neo.08582

Figure 1.

Figure 1

(A) Correlation between PTEN and AR expression in PC and increased frequency of high expressors of PTEN and AR in AD PC. Immunohistochemistry LI of PTEN and AR were directly (continuous line) correlated in ADPC (closed circle) and AIPC (open circle; rs = 0.6604, P = .0015 and rs = 0.6320, P = .0065). Dotted lines passing through the median value of each IHC LI identify the region where most ADPC and only a few of AIPC cases are observed (high expressors). Most of AIPC cases are scattered outside this area. (B and C) Expression of AR (right) and PTEN (left) in serial sections of PC. Photomicrographs show adjacent PC areas where AR and PTEN are differentially expressed. Cytoplasmic expression of PTEN is associated with strong AR nuclear reactivity. In areas where PTEN is negative, AR is confined to the cytoplasm (original magnification, x200). (D–G) DU145 and LNCaP as controls for PTEN and AR antibodies. DU145 cells expressed cytoplasmic PTEN (D) but were AR-negative (E). Conversely, nuclear expression of AR was observed in LNCaP cells (G), whereas PTEN was absent (F). (H) Kaplan-Meier curve showing relapse-free survival in ADPC patients stratified by PTEN and AR coexpression levels. High expressors and low expressors are represented by a continuous and an interrupted line, respectively. Log rank test was used to compare curves. P < .05 was considered significant. Time until disease recurrence (relapse-free survival) was measured from the date of surgery until the development of metastases and/or bladder outlet obstruction (clinical relapse) or three consecutive increases in the PSA level greater than the nadir (biochemical relapse). At 30 months after surgery, the probability of relapse-free survival for high expressors of PTEN and AR among AD patients was 85.71% (SEM = 9.35) compared to only 16.67% (SEM = 15.21) in low expressors. At 36 months, only 28.57% (SEM = 9.35) of high expressors had experienced a biochemical relapse compared to 100% of low expressors (hazard ratio, 4.65; 95% CI, 4.719–146.8).