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. 2008 Sep;10(9):1004–1013. doi: 10.1593/neo.08548

Figure 1.

Figure 1

The involvement of JNK/SAPK signaling in mammary gland development. Sections of mouse virgin gland (A–E) and mammary gland undergoing pregnancy-dependent morphogenesis (F–J) were analyzed by immunochemical staining for phosphorylated JNK (PJNK; A, F), phosphorylated p38 (Pp38; B, G), and phosphorylated Erk1/2 (PErk1/2; C, H), phosphorylated c-Jun at Ser63 (PJun63; D, I) and phosphorylated c-Jun at Ser73 (PJun73; E, J). In the virgin gland, mammary epithelia showed that PErk1/2 is highly expressed and PJun73 is positive for a few cells. In contrast, all JNK/SAPK signaling molecules were strongly activated during pregnancy-mediated mammary development. The immunochemistry (brown)-stained sections were counterstained with hematoxylin (blue). The experiments were performed in three different wild type and transgenic animals (n = 3). Scale bar, 100 µm (A–J).