Figure 2.

JNK/SAPK signaling is not induced in mammary hyperplasia caused by elevated levels of Wnt/β-catenin signaling. Sections of the wild type (A–F) and hyperplastic (G–L) mammary glands caused by high levels of Wnt signaling were analyzed by immunochemistry for the expression of β-catenin (β-cat; A, G), PJNK (B, H), Pp38 (C, I), PErk1/2 (D, J), PJun63 (E, K), and PJun73 (F, L). The insets in A and G display magnified images revealing differential localization of β-catenin. The immunochemistry (brown)-stained sections were counterstained with hematoxylin (blue). The experiments were performed in four different wild type and transgenic animals (n = 4). Scale bars, 100 µm (A–I).