Table 1.
Selection of Membrane Proteins Identified in the MDA-MB-231 Cell Line (MDA, n = 4) and MDA-MB-231-B02 Cell Line (B02, n = 4).
| Name | MDA | SC | BO2 | SC | Biologic Process | Cell. Component |
| CD51 (integrin αv) | 1 | 3 | 3 | 4 | Cell comm., Signal transd. | PM |
| CD9 (MRP-1) | 1 | 1 | 4 | 5 | Immune resp. | PM |
| Plasma cell membrane glycoprotein PC1 | 1 | 1 | 4 | 13 | Metabolism energy pathways | PM |
| Kinectin | 1 | 4 | 4 | 19 | Cell growth and/or maintenance | ER, L |
| Prohibitin | 1 | 7 | 4 | 27 | Cell comm., Signal transd. | MC, PM, N, EC, C |
| Moesin | 3 | 35 | 3 | 26 | Cell growth and/or maintenance | PM, C, N |
| EGF receptor | 4 | 12 | 4 | 26 | Cell comm., Signal transd. | PM, C |
| CD49b (integrin α2) | 4 | 19 | 4 | 15 | Cell comm., Signal transd. | PM, C |
| Integrin β6 | 4 | 22 | 4 | 13 | Cell comm., Signal transd. | PM |
| Clathrin, heavy polypeptide | 4 | 47 | 4 | 22 | Cell growth and/or maintenance | C, PM, G |
| CD44 | 4 | 61 | 4 | 29 | Cell comm., Signal transd. | - |
| CD98 | 4 | 68 | 4 | 68 | Transport | PM |
| CD29 (integrin β1) | 4 | 162 | 4 | 60 | Cell comm., Immune cell migr. | PM |
| HLA-A | 4 | 600 | 4 | 51 | Immune resp. | PM, ER, EC |
| CD49c (Integrin α3) | 4 | 24 | 3 | 4 | Cell comm., Signal transd. | PM, C |
| CD155 (PVR) | 1 | 1 | 0 | 0 | Cell comm., Signal transd. | PM, EC |
| CD104 (integrin β4) | 1 | 7 | 0 | 0 | Cell comm., Signal transd., Cell migration, Cell adhesion | ITM |
| CD107b (LAMP2) | 2 | 2 | 1 | 1 | Cell growth and/or maintenance | EC, ER, PM, G |
| CD49e (integrin α5) | 3 | 8 | 1 | 1 | Cell comm., Signal transd. | PM |
| CD97 | 3 | 9 | 1 | 1 | Cell comm., Signal transd. | - |
| CD49f (integrin α6) | 4 | 21 | 1 | 1 | Cell comm., Signal transd. | PM |
| HLA-C | 4 | 374 | 0 | 0 | Immune resp. | PM |
| HLA-B | 4 | 431 | 1 | 19 | Immune resp. | PM, EC |
The proteins are identified from the SwissProt database with Mascot. The numbers in bold indicate in how many runs each protein was detected. Function and location of the proteins are determined using the Human Protein Reference Database (www.hprd.org).
C indicates cytoplasm; Cell comm., cell communication; EC, extracellular; ER, endoplasmic reticulum; G, Golgi; Immune cell migr., immune cell migration; Immune resp., immune response; L, lysosome; MC, mitochondrion; N, nuclear; PM, plasma membrane; SC, spectral counting (italicized numbers represent the number of redundant spectra identifying the same protein or group of protein, e.g. HLA-A, -B, and -C serotypes); Signal transd., signal transduction.