Figure 3. IL-10 expression following treatment of HIV-infected PBLs with skTRAIL, a representative example of results obtained from assays with the Bio-Plex human cytokine 27-plex panel.

PBLs were collected from 4 HIV-infected donors and 1 uninfected control. Cells received either no treatment or were treated with either BSA (5 ng/ml) or skTRAIL (5 ng/ml). Following 24 h of culture, supernatants were collected for measurement of cytokine expression. Values are presented as relative fluorescence units, reflecting a range of concentrations from 0.08 to 2.01 pg/ml based on extrapolation from a standard curve generated with known concentrations of IL-10 at the time of analysis.