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Histopathological analysis of heart in perforin (+/+) (a, c and e) and (−/−) mice (b, d and f). Hematoxilin/eosin-stained heart sections were taken after 8 (a and b), 15 (c and d), and 22 (e and f) days post infection with Y strain T. cruzi. The original magnification was 160×.