Figure 6.

(a) Changes in wet weight of mouse gastrocnemius muscle after intramuscular injection of 100 µg T. nattereri venom dissolved in 100 µL PBS. At various time intervals mice were sacrificed and both injected and contralateral muscles were excised and weighed. Results are expressed as a percentage, taking as 100% the weight of contralateral, noninjected muscle. (b) CK content of mouse gastrocnemius muscle at different time intervals after intramuscular injection of 100 µg T. nattereri venom dissolved in 100 µL PBS. Both injected and contralateral gastrocnemius muscles were homogenized and, after centrifugation, CK activity of the supernatant was quantified. CK activity in envenomated muscle is expressed as a percentage, taking as 100% the CK activity of contralateral, noninjected gastrocnemius. Results are presented as mean ± SD (n = 5).