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Medium from ND-1 cell cultures used in MMP-9 CAT assays (Figure 3) was further analyzed for TGF-β peptide levels (A) and type IV collagenase activity (B). Lane 1, control samples (transfected with pBabeNeo); lane 2, treated with exogenous TGF-β1; lane 3, transfected with pBabe TGF-β1Neo; lane 4, transfected with pBabeRasNeo (as positive control).
