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Western blot analysis of cell-associated MMP-9 levels in the absence and presence of actinomycin D or cycloheximide. ND-1 cell (A) and TsuPr1 cell (B) extracts were collected 24 h after treatment with TGF-β1, subjected to 7.5% SDS-PAGE, and immunoblotted using a polyclonal anti-MMP-9 antibody.
