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Analyses of MMP-2 regulation by TGF-β1 in the TsuPr1 cell line. MMP-2 levels were analyzed after TGF-β1 stimulation for 24 h by Northern blot (A), immunoblotting of intracellular and secreted proteins (B), or zymography of secreted 72-kDa gelatinase activity (C) present after 0, 1, or 2 d of cell-free incubation at 37°C.
