Figure 1.
Production of NO by spleen cells of JEV-inoculated mice. Groups of mice were inoculated with JEV intraperitonially (i.p.). Spleens were harvested at different time periods, a single cell suspension was prepared (10 × 106cells/ml) and cultured for 24 h at 37 °C. NO production was assayed in the cell free culture supernatents (▪) as described in Materials and methods. Control (□) mice were inoculated with normal mouse brain suspension. Values are presented as A. M. ± SD from 10 cultures.