Figure 1.
Immunoblot after SDS/PAGE of several tissues of wildtype (odd numbered lanes) and transgenic mice (even numbered lanes). Tissues in lanes are: 1,2: heart; 3,4: kidney; 5,6: lung; 7,8: liver; 9,10: spleen; 11,12: skeletal muscle; 13,14: brain; 15,16: uterus, and 17,18: spinal cord, with different expression levels of αA-crystallin and an elongated form αAins-crystallin of approximately 19.8 and 22.5 kDa, respectively. Detection was achieved with an affinity-purified anti-αA-crystallin antibody.