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Number of parasites (mean ± SE) per footpad in BALB/c mice injected with 107Leishmania (L.) amazonensis promastigotes pre-incubated with 50 ng/ml of IGF-I. Control mice were infected with nonactivated parasites. Number of parasites was quantified using the limiting dilution assay as given in the Materials and Methods. (a) 7 days postinfection; (b) 60 days postinfection. *P ≤ 0.05 (Mann–Whitney test).
