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Number of parasites per cell (mean ± SE) from skin lesions in BALB/c mice injected with 107Leishmania (L.) amazonensis promastigotes pre-incubated with 50 ng/ml of IGF-I (solid column). Control mice were infected with nonactivated parasites (open column). For further details see Materials and Methods. *P ≤ 0.05 (Mann–Whitney test).
