Figure 2.

Immunohistochemistry. (a) Pancreas of untreated animal. Ducts are widely separated by closely packed keratin-negative acini. (Cytokeratin). (b) Pancreas 1 day after commencement of caerulein injections. Relatively more tubular complexes. (bcl-2). (c) Pancreas 4 days after commencement of caerulein injections. Tubular complexes with few keratin negative epithelial cells present. (Cytokeratin). (d) Pancreas 7 days after commencement of caerulein injections. Nearly normal. (bcl-2). (e) Pancreas 2 days after commencement of caerulein injections. Note numerous small darkly staining amylase positive apoptotic bodies (dark specks) within epithelium and mainly single residual acinar cells (arrows). (Double staining for amylase (dark) and cytokeratin (light)). (f) Pancreas 4 days after commencement of caerulein injections. Note absence of the amylase positive apoptotic bodies seen in (e) and clustered amylase positive acinar cells, some seen as demilunes surrounding lightly staining complexes lined by duct-like cells (arrows). (Double staining for amylase (dark) and cytokeratin (light)). (a, b, c, d, bar = 50 μm; e, f bar = 25 μm; counterstain haematoxylin).