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. 1999 Aug;80(4):189–196. doi: 10.1046/j.1365-2613.1999.00114.x

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© 1999 Blackwell Science Ltd

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Percentage of TUNEL positive cells in the cardiac myocytes treated with CT-1 and the various signal transduction inhibitors 30 min prior to exposure to simulated lethal hypoxia/ischaemia (I). I + CT-1 (); I + CT-1 in the presence of 50μm PD98059 (▪) or 10μm SB203580 (). Untreated cardiac myocytes (□). The cells were fixed using 4% paraformaldehyde and the percentage of TUNEL positive nuclei were analysed using fluorescein-12 dUTP and terminal transferase. Values are the means of three determinations whose S.E.M. is shown by the bars. *P < 0.05.

Inline graphic — Percentage of TUNEL positive cells in the cardiac myocytes treated with CT-1 and the various signal transduction inhibitors 30 min prior to exposure to simulated lethal hypoxia/ischaemia (I). I + CT-1 (); I + CT-1 in the presence of 50μm PD98059 (▪) or 10μm SB203580 (). Untreated cardiac myocytes (□). The cells were fixed using 4% paraformaldehyde and the percentage of TUNEL positive nuclei were analysed using fluorescein-12 dUTP and terminal transferase. Values are the means of three determinations whose S.E.M. is shown by the bars. *P < 0.05.