Figure 1.

Immunofluorescence analysis of the intracellular localization of eps15 in NIH-EGFR transfectants. (a) The pattern of staining with anti-eps15 antibody is punctate and the small dots are localized either at the cell periphery and along cell projections (arrowheads) or concentrated in the perinuclear Golgi area (arrows). (b) After treatment with nocodazole, resulting in microtubule depolymerization and subsequent Golgi fragmentation, the dots appear dispersed from the perinuclear cluster. The disrupting effect of nocodazole on microtubules was established by double staining with anti-tubulin antibodies. Bar, 5 μm. The anti-eps15 staining in this and all subsequent experiments was obtained with the described affinity-purified anti-eps15 antibody. In addition, comparable results were obtained using anti-eps15 monoclonal antibodies).