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Kinetic Constants for TvPNP with Various Substrates
| Substrate | Km (μM) | kcat (s-1) | kcat/Km (M-1 s-1) |
|---|---|---|---|
| Adenosine | 6.1 ± 0.5 | 1.7 ± 0.2 | 2.78 × 105 |
| Guanosine | 59.7 ± 8.1 | 1.1 ± 0.1 | 1.8 × 104 |
| Inosine | 25 ± 1 | 1.8 ± 0.1 | 7.2 × 104 |
| pNPR | 90 ± 42 | 1.0 ± 0.1 × 10-3 | 1.1 |
