Table 4.

Immucillin Binding and Release Constants

constant	ImmA	DADMe-ImmA
Ki	1,000 pM	1,500 pM
Ki*	87 pM	30 pM
k3a	7.0 × 10-3 s-1	8.8 × 10-3 s-1
K4b	6.7 ± 1.0 × 10-4 s-1	1.8 ± 0.5 × 10-4 s-1
t1/2 on	99 s	79 s
t1/2 off	17.2 min	64 min

^aValues of k₃ were estimated using K_i,K_i*, and k₄ (see footnote ²).

^bValues of k₄ were determined experimentally from Figure 2B.