Figure 11.

More Sec22b/ERS-24 antibodies are required for inhibition of transport of 15°C-arrested G protein from the IC to the Golgi. (A) Transport reactions using semi-intact cells in which G protein has been arrested in the ER (square) or the IC (diamond) (by preincubating cells in vivo for 90 min at 15°C before the preparation of semi-intact cells) were incubated for the indicated time at 32°C. In each transport, the final rate of G protein transported to Golgi was set as 100%. (B) In vitro transport from the IC to the Golgi was performed either on ice (lane 1) or at 32°C (2–6) in the absence (lane 3) or presence (lane 1, 2, 4–6) of rlc. The reagents were omitted or added as indicated. (C) In vitro IC–Golgi transport, supplemented with the indicated amounts of Sec22b/ERS-24 antibodies, was performed at 32°C. (D) In vitro ER–Golgi transport was performed at 32°C supplemented with the indicated amounts of Sec22b/ERS-24 antibodies.