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NRK cells under normal conditions (a and b), or treated with 10 μg/ml brefeldin A for 1 h (d and e), or treated with 10 μg/ml nocodazole for 1 h (g and h) were fixed and double-labeled for Sec22b/ERS-24 (a, d, and g) and Golgi mannosidase II (man II) (b, e, and h). The merged images are also shown (c, f, and i). Bar, 10 μm.
