NRK cells under normal conditions (a and b), or treated with 10 μg/ml brefeldin A for 1 h (d and e), or treated with 10 μg/ml nocodazole for 1 h (g and h) were fixed and double-labeled for Sec22b/ERS-24 (a, d, and g) and KDEL R (b, e, and h). The merged images are also shown (c, f, and i). Bar, 10 μm.