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NRK cells were incubated at 15°C for 3 h and then incubated at 37°C for the indicated time. The cells were fixed and double labeled for Sec22b/ERS-24 (a, d, g, and j) and KDEL R (b, e, h, and k). The merged images are also shown (c, f, i, and l). Bar, 10 μm.
