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. 1999 Feb;10(2):435–453. doi: 10.1091/mbc.10.2.435

Figure 7.

Figure 7

Sec22b/ERS-24 antibodies specifically inhibit in vitro ER–Golgi transport. (A) In vitro ER–Golgi transport was performed either on ice (lane 1) or at 32°C (lanes 2–8) supplemented with the indicated amounts of Sec22b/ERS-24 antibodies. (B) In vitro transport was performed either on ice (lane 1) or at 32°C (lanes 2–4) supplemented with 3 μg of Sec22b/ERS-24 antibodies (lane 3) or heat-inactivated antibodies (lane 4). (C) In vitro transport was performed either on ice (lane 1) or at 32°C (lanes 2–7) supplemented with indicated amounts of GST-Sec22b. (D) In vitro transport was performed either on ice (lane 1) or at 32°C (lanes 2–5) in the absence (lane 3) or the presence of rat liver cytosol (rlc) (lanes 1, 2, 4, and 5) supplemented with 2 μg of Sec22b antibodies (lane 4), or 2 μg of Sec22b antibodies and 1.5 μg of GST-Sec22b (lane 5). (E) In vitro transport was performed at 32°C in the absence (lane 1) or the presence of indicated amounts of the Fab fragment of Sec22b antibodies (lanes 2–9).