Skip to main content
. 1999 Feb;10(2):435–453. doi: 10.1091/mbc.10.2.435

Figure 8.

Figure 8

Sec22b/ERS-24 antibodies must be present before the EGTA-sensitive stage to exhibit the inhibitory effect on in vitro ER–Golgi transport. In vitro ER–Golgi transport was performed at 32°C in the absence (lane 1) or presence of rat liver cytosol (rlc) (lanes 2–9). For lanes 3, 5, 7, and 9, transport assay was first performed in the presence of 12.5 mM EGTA to arrest the transport at the EGTA-sensitive stage followed by a washing step and second incubation at 32°C to continue the transport. Reagents were supplemented as indicated.