Skip to main content
. Author manuscript; available in PMC: 2008 Aug 19.
Published in final edited form as: Langmuir. 2007 Apr 11;23(10):5817–5823. doi: 10.1021/la063659c

Figure 4.

Figure 4

(a) Specific detection of target rabbit IgG (1 mg ml-1) with a biotinylated anti-rabbit IgG receptor (green triangle) and non-specific binding of rabbit IgG to biotinylated anti-chicken IgG receptor (red diamond) for varying concentrations of receptor molecules. ([Biotin-LC-LC-NHS]=0.5 mg ml-1; [SA]=0.07mg ml-1). (b) Time study of rabbit IgG (0.25 mg ml-1) binding to optimized linking chemistry (biotin-LC-LC-NHS=0.5 mg ml-1, SA=0.07 mg ml-1, B-Ab=0.07 mg ml-1). (c) Rabbit IgG calibration curve (biotin-LC-LC-NHS=0.5 mg ml-1, SA=0.07 mg ml-1, B-Ab=0.07 mg ml-1, rabbit IgG concentration varied) (a) - (c) All concentrations refer to solution concentrations applied to sensor.