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. Author manuscript; available in PMC: 2008 Aug 19.
Published in final edited form as: Biochemistry. 2007 Mar 20;46(14):4250–4260. doi: 10.1021/bi602422p

FIGURE 1.

FIGURE 1

SDS-PAGE of wt DevS642 (molecular weight 25330 Da) and H149A mutant after purification by affinity chromatography. In each lane about 0.5 μg of protein was loaded on a 12% BT Novex NuPage gel. Lane 1, SeeBlue Plus 2 prestained protein standard (7 μL); lane 2, DevS642 expressed in the presence of hemin, lane 3, H149A mutant expressed in the presence of hemin; lane 4, DevS 642 apoprotein; lane 5, H149A mutant apoprotein.