Table 3. Kinetic analysis of zebrafish IGFBP-1a, -1b and human IGFBP-1 interactions with IGF biosensor surfaces.

IGF-1
	ka(×105 M−1×s−1)	kd(×10−4 s−1)	KD(nM)	χ2	Relative KD
zebrafish IGFBP-1a	11.80±0.040	3.32±0.007	0.28	0.2	1.0
zebrafish IGFBP-1b	1.71±0.004	20.60±0.053	12.0	0.202	42.8
human IGFBP-1	0.53±0.001	3.14±0.008	5.96	0.032	21.2

Association rate (k_a), dissociation rate (k_d), and dissociation constants (K_D: k_d/k_a) are given.