Skip to main content
. 2008 Aug 29;3(8):e3106. doi: 10.1371/journal.pone.0003106

Figure 1. Identification of a Giardia U1-snRNA candidate.

Figure 1

A. Proposed structure for writing the U1 descriptor file. The content in the U-1 descriptor cell can be visualized in this figure. “s” stands for strand and “h” stands for helix. The elements within the proposed U-1 structure are marked in order from the 5′-end to the 3′-end. The two stem-loops drawn as dotted lines are not compulsory in the proposed structure of Giardia U-1 candidate; therefore they are marked as a free-folding strand s4. B. The structures of Human, T. brucei and Giardia-candidate U1-snRNAs. The conserved loops among the human, Giardia and Trypanosome U1-snRNAs are indicated by the circles. The Sm-protein-binding sites are boxed. C. RT-PCR test showing high expression the of the Giardia U1-snRNA candidate. + control: PCR with genomic DNA. − control: PCR with total RNA without reverse transcription.