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. 2008 Aug 27;3(8):e3077. doi: 10.1371/journal.pone.0003077

Figure 9. Increased expression of chemokine receptors (CXCR1, 2) in lung CSCs.

Figure 9

A, Immunofluorescent images of CXCR1 and CXCR2 in H460 and CS cells. H460 cells and lung CSCs dissociated from spheres were plated into 96-well plates precoated with Collagen IV and cultured 8 h. Then adherent cells were incubated with antibodies against CXCR1 and CXCR2 and with secondary antibodies conjugated with Alexa Fluor® 488 and stained with Hoechst33342. Images were acquired using the Cellomics ArrayScan HCS Reader (20X objective) and analyzed using the Target Activation BioApplication Software Module. B, Fluorescence intensity (pix) of CXCR1 and CXCR2 plotted against object area.