Table 2. Multiplex analysis of cytokines and growth factors in the lysates of xenografted parental H460 and CSC-derived tumors.

Tumor Producing Factors		Mean±SE pg/mg of protein
Cytokines		H460-derived tumor	CSCs-derived tumor	P value
1	IGFBP-1	18,853±1,583	62,090±6,210	<0.001
2	VEGF	3,218±516	8,249±980	<0.001
3	IL-8	6,295±905	10,360±700	<0.05
4	IL-6	1,808±184	3,599±479	<0.05
5	bFGF	941±84	3,055±657	<0.01
6	HGF	183±24	413±31	<0.001
7	PDGF-BB	8±1	24±6	<0.05
8	SCGF-β	1015±149	16,599±4,802	<0.001
9	SDF-1α	197±38	895±85	<0.05
10	SCF	61±4	80±1	<0.05
11	G-CSF	15±1	344±22	<0.001
12	GM-CSF	13±2	28±4	<0.01
13	IFNα2	94±13	203±27	<0.05
14	MIP-1α	18±1	38±5	<0.01
15	MCP-1	6±0.5	15±2	<0.01
16	MIG	8±0.8	16±1	<0.05
17	PAI-1	459±25	1,546±142	<0.01
18	TNFα	48±9	94±9	<0.05
19	TRAIL	116±23	231±23	<0.05

Sonicated extracts were prepared from H460- and CSC-derived tumors growing in SCID mice (5 tumors per group) and concentrations of various tumor-producing cytokines, chemokines, angiogenic and growth factors were analyzed using multiplex kits. Only factors with significant differences in their concentrations (at least p<0.05) are included.