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. 2008 Aug 29;3(8):e3099. doi: 10.1371/journal.pone.0003099

Figure 1. Fluorescence microscopy.

Figure 1

All figures are from CHP-212. A–F. Combined immunofluorescence (IF) for beta tubulin (red), fluorescence in situ hybridisation (FISH) with a MYCN probe (green), and chromatin counterstaining by DAPI (blue) shows translocation of DMs to the periphery of the chromosome rosette at transition from interphase (A) to prometaphase (B); the peripheral localisation is maintained through metaphase (C), anaphase (D) and telophase (E), after which DMs again transfer to interior positions in the nucleus (F). G–J. Serial confocal optical sections (i–vi) corroborate the peripheral positions of DMs (green) at prometaphase (G), metaphase (H), and telophase (I), in contrast to a more central localisation at interphase (J); chromatin is counterstained by DRAQ5 (red). K. Proportion of peripheral DMs at prometa/metaphase, ana/telophase, and interphase; error bars denote standard deviation. L. The proportion of DM (MYCN) signals overlapping with telomeric TTAGGG-repeat signals at mitosis and interphase, respectively, in LA-N-5 and CHP-212. M–N. FISH for telomeric TTAGGG sequences (green) and MYCN (red) shows more frequent overlapping of signals for telomeres and DMs at metaphase (M, right) and telophase (N), compared to interphase (M, left).