Figure 3.

Staining of coiled bodies with anti-SLBP1 serum X16C is reduced by treatment with peptide-1, against which the antibody was raised. (A) Images of stained coiled bodies were taken with a charge-coupled device (CCD) camera; the total fluorescence from individual coiled bodies was then measured as the sum of pixel values and plotted as a function of coiled body volume. The linear relationship shows that the amount of SLBP1 in a coiled body is proportional to its volume. Staining was markedly reduced by previous treatment of the antibody with peptide-1, whereas the unrelated peptide-2 had no effect. (B) The slopes of the curves in A were determined and compared. The slope represents the amount of stain per unit volume and is assumed to be a measure of stain per unit of SLBP1.