Figure 5.

Transcripts of full-length myc plus NLS–tagged SLBP1 were injected into the oocyte cytoplasm. Sixteen hours later, GVs and cytoplasms (cyt) were manually isolated, and their proteins were separated on an SDS-polyacrylamide gel. Western blots were made with mAb 9E10 against the c-myc tag (lanes 1 and 2) or with antibody X1 against SLBP1 (lanes 3 and 4). All myc-tagged SLBP1 is found in the nucleus and is recognized by both antibodies at a mobility corresponding to ∼50 kDa. Antibody X1 also recognizes endogenous SLBP1 in both nucleus and cytoplasm at a mobility of ∼40 kDa. It also stains a minor cross-reacting band in both nucleus and cytoplasm just below the heavy band of myc-SLBP1.