Figure 1.

Tissue-Specific Expression of Two Types of Phosphorylase in Rice.

(A) Activities of two starch Pho isoforms, Pho1 and Pho2, were detected by native-PAGE/enzymatic activity staining analysis in various tissues. The identification of Pho1 and Pho2 was based on our previous study (Yamanouchi and Nakamura, 1992). Lane 1, cv Kinmaze; lane 2, cv Taichung 65 (T65); lane 3, EM583 (a wx mutant derived from the MNU treatment of T65).

(B) SDS-PAGE analysis of Pho1 excised from the native-PAGE gel from rice developing endosperm.

(C) Primary sequences of a tryptic peptide from the Pho1 protein obtained by liquide chromatography–tandem mass spectrometry analysis and alignment with the unique insertion sequences of Pho1 reported in the database (NCBI nr).