Figure 5.

Schematic Representation of Mutations in the Pho1 Gene of BMF136 and EM640.

(A) Schematic representation of the Pho1 gene and the locations of single nucleotide substitutions in pho1 mutant lines BMF136 and EM640. ATG and TAG indicate the initiation and termination codons, respectively. Gray boxes, lines, and arrowheads indicate exons, introns, and mutation sites, respectively. a.a., amino acid.

(B) Alignment of the Pho1 gene from T65 with the pho1 gene from the BMF136 mutant. The splice site border sequence between the fifth exon and intron (at nucleotide 2626) was disrupted by a G-to-A mutation, which results in intron retension and generates a stop codon 66 nucleotides downstream of the mutation site.

(C) Alignment of the Pho1 gene from T65 with the pho1 gene from the EM640 mutant. In EM640, a C-to-T transition occurred at nucleotide 4716 in the tenth exon, and this resulted in a codon change from CAG (Gln) to a stop codon (TAG).