Figure 6.

Effects of pho1 Mutations on Activities of Starch Biosynthetic Enzymes.

Native-PAGE/activity staining analysis was performed using enzymes from three developing endosperms of pho1 mutants BMF136 and EM640 and of their wild-type parent, T65. The numbers above the lanes indicate the volume (μL) of crude enzyme extract applied to each gel lane. The activity bands for isoamylase, pullulanase, and Pho1 (A), for branching enzymes, BEI, BEIIa, and BEIIb, and for Pho1 (B), and for SSI and SSIIIa (C) are indicated with arrowheads. Note that the activity bands for BEIIb and Pho1 are not resolved and overlap on the polyacrylamide gel as denoted by the asterisk in (B), as previously described (Yamanouchi and Nakamura, 1992). Note that the staining intensity of this band is mainly due to Pho1 activity, which is absent in BMF136 and EM640.