Table 3.
Frequency of Four Grain Phenotypes in pho1 Mutant Seeds Developed under Three Different Temperature Conditions
| Exp. 1 (EM755)
| ||||||
|---|---|---|---|---|---|---|
| 30°C
|
25°C
|
20°C
|
||||
| Type | No. (%) | G.W. (mg) | No. (%) | G.W. (mg) | No. (%) | G.W. (mg) |
| T65 | 20 (100) | 22.8 ± 1.2 | 20 (100) | 22.3 ± 1.1 | 20 (100) | 24.8 ± 0.8 |
| EM755 | ||||||
| pn | 20 (39) | 20.2 ± 1.2 | 16 (18) | 22.0 ± 1.3 | 12 (16) | 21.4 ± 1.2 |
| wcg | 29 (57) | 19.6 ± 2.8 | 42 (47) | 23.8 ± 1.1 | 12 (16) | 20.6 ± 1.0 |
| shr | 2 (4) | 10.8 ± 0.2 | 31 (35) | 5.6 ± 0.9 | 23 (32) | 9.5 ± 2.5 |
| S-shr | 0 (0) | 0 (0) | 26 (36) | 4.4 ± 0.7 | ||
| Total | 51 (100) | 89 (100) | 73 (100) | |||
| Exp. 2 (BMF136)
| ||||||
| 30°C
|
25°C
|
20°C
|
||||
| Type | No. (%) | G.W. (mg) | No. (%) | G.W. (mg) | No. (%) | G.W. (mg) |
| T65 | 30 (100) | 22.4 ± 1.8 | 30 (100) | 22.5 ± 2.1 | 30 (100) | 22.9 ± 1.7 |
| BMF136 | ||||||
| pn | 18 (17) | 20.9 ± 3.4 | 26 (24) | 22.1 ± 1.1 | 2 (3) | 19.5 ± 0.3 |
| wcg | 82 (77) | 18.4 ± 3.7 | 40 (37) | 22.7 ± 2.5 | 15 (19) | 20.5 ± 1.3 |
| shr | 6 (6) | 10.0 ± 1.2 | 41 (39) | 6.6 ± 1.5 | 24 (31) | 7.9 ± 1.4 |
| S-shr | 0 (0) | 0 (0) | 36 (47) | 3.6 ± 1.0 | ||
| Total | 106 (100) | 107 (100) | 77 (100) | |||
G.W., grain weight; pn, pseudonormal grain; wcg, white-core endosperm grain; shr, shrunken grain; S-shr, severely shrunken grain.