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Sucrose Supplementation to Excised Siliques Saves Embryo Development in Photorespiratory Mutants.
Excised surface-sterilized wild-type, dKO, and shm1-1 mutant siliques carrying globular embryos were cultured in the presence or absence of 3% sucrose on Murashige and Skoog medium for 7 d. Seeds were cleared in Hoyer's solution for Nomarski microscopy, or the embryos were dissected directly from the seeds.
