Figure 9.

RNA Gel Blot Analysis of Stress-Inducible Genes under Different Stress Conditions.

(A) The transcripts of BiP and bZIP60 were induced to higher levels in lew1 than in the wild type by drought stress. Four-week-old seedlings were removed from soil and placed on a laboratory bench for 1 h (drought 1 [Dro1]). Then, seedlings were covered with a transparent film (to slow water loss) for 1 or 4 h (drought 2 h and 5 h [Dro2 and Dro5, respectively]). Total RNA from each treatment was used for RNA gel blot analysis. RNA from shoots without treatment was used as a control at 0 h (Con). rRNAs stained with ethidium bromide were used as loading controls.

(B) More BiP transcripts accumulated in lew1 than in the wild type under mannitol treatment. Four-week-old seedlings from soil were dipped into solution containing 300 mM mannitol for 1, 2, or 6 h (Man1, Man2, and Man6, respectively), and total RNA was analyzed by RNA gel blot. rRNAs stained with ethidium bromide served as loading controls. RNA from shoots without treatment was used as a control at 0 h (0).

(C) The transcripts of RD29A and COR47 were induced to higher levels in lew1 than in the wild type by drought stress. Drought treatment was done as in (A). rRNA was used as a loading control.

(D) Four-week-old seedlings were treated with 150 mM NaCl for 0.5 h (NaCl0.5) or 1 h (NaCl1) or with 300 mM mannitol for 1 h (Man1) or 2 h (Man2). rRNAs stained with ethidium bromide served as loading controls. RNA from shoots without treatment was used as a control at 0 h (Con).

(E) Two-week-old seedlings grown on MS medium were treated with 0.1 μg/mL tunicamycin for different times, and total RNA was analyzed by RNA gel blot. rRNAs stained with ethidium bromide served as loading controls.