Figure 1.

Basal and Induced Expression of the TPK1b Gene.

RNA gel blot analysis showing upregulation of TPK1b gene expression by

(A) B. cinerea.

(B) P. syringae.

(C) Mechanical wounding.

(D) Oxidative stress (paraquat, methyl viologen).

(E) Basal expression in fruit and leaf tissues.

Total RNA (15 μg) was loaded per lane. rRNA staining is shown as a loading control. The experiments were repeated at least three times with similar results. Wild-type plants of the tomato cultivar CastlemartII were inoculated with pathogens or were treated with chemicals as described in Methods. h, h after inoculation or chemical treatment; L, leaf; GF, green fruit; GFR, green–red fruit; RF, red fruit; S, stem.