Figure 7.

Expression of Tomato Defense-Related Genes in Wild-Type and TPK1b RNAi Tomato Plants.

(A) and (B) RT-PCR showing the expression of tomato PI-II during Botrytis infection or mechanical wounding (A) and ACC or MeJA treatment (B).

(C) and (D) RNA gel blot showing the expression of tomato ACC synthase and PR-1 during Botrytis infection (C) and mechanical wounding (D).

(E) Expression of allene oxide synthase2 during Botrytis infection or mechanical wounding.

In (C) to (E), total RNA (15 μg) was loaded per lane, and rRNA is shown as a loading control. Experiments were repeated at least three times with similar results. h, h after Botrytis inoculation or wounding treatment; ACS, ACC synthase; AOS2, allene oxide synthase2.