Figure 1.

WRKY3 Strongly Responds to Wounding, and WRKY6 Requires M. sexta's OS for Transcript Elicitation.

(A) Alignment of WRKY3 and WRKY6 proteins. Identities of both proteins are shaded with black, and conserved WRKY domains are enclosed in the black box.

(B) Mean (±se) WRKY3 transcript levels in elicited (filled symbols) and unelicited (systemic; open symbols) leaves in five replicate N. attenuata wild-type plants at each harvest time and treatment. Leaves were elicited by wounding and immediately treating the puncture wounds with 20 μL of water (W; dotted lines) or 1:1 diluted OS from M. sexta larvae (solid lines). Inset: induction of WRKY3 transcripts in an independent experiment using wounding and water (W; dotted line), 1:1 diluted OS (solid line), or a synthetic mixture of the two most abundant FACs in M. sexta OS, N-linolenoyl-l-glutamine and N-linolenoyl-l-glutamate (FAC; dashed line). Transcripts were analyzed by real-time PCR in arbitrary units after calibration with a 4× dilution series of cDNAs prepared from RNA samples containing WRKY3 or WRKY6 transcripts.

(C) WRKY6 transcript levels determined as above. The x axes in (B) and (C) are on a nonlinear scale to reveal the culmination of transcript levels between 0 and 60 min.