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. 2008 Jul;20(7):1984–2000. doi: 10.1105/tpc.108.058594

Figure 2.

Figure 2.

WRKY3 Is Required for WRKY6 Transcript Accumulation, and Both Are Required for Resistance to M. sexta Larvae.

(A) Mean (±se) WRKY3 basal transcript levels in five replicates of each wild-type, WRKY3-, and WRKY6-silenced plants. Transcripts were analyzed by real-time PCR. Different letters reflect significant differences at P < 0.05 from wild-type plants of the same treatment.

(B) Mean (±se) WRKY6 basal transcript levels in five replicates of each wild-type, WRKY3-, and WRKY6-silenced plants as above.

(C) Mean (±se) mass of M. sexta larvae that fed on wild-type (solid line), two lines of WRKY3-silenced (ir-wrky3; dotted lines), and two lines of WRKY6-silenced (ir-wrky6; dashed lines) N. attenuata plants in the glasshouse for 8 d (n = 12/genotype).

(D) Mean (±se) of JA and JA-Ile/-Leu concentrations and transcript levels of LOX3 after M. sexta larvae fed on the plants. FM, fresh mass.

(E) Mean (±se) of TPI activity measured 4 d after M. sexta larvae fed on the plants. Different letters reflect significant differences at P < 0.05 from wild-type plants of the same treatment.